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Contrary to oligo(dT) primers, these primers aren't template unique and are suited to DNA synthesis working with Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates.
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Swimming pools of pre-intended primers may help generate extensive expression profiles, In particular exactly where assay sensitivity is critically crucial.
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Magnum masses call for much more activation Vitality due to the fact You will find there's great deal a lot more powder to burn up. Occasionally, a primer could be formulated to operate with both equally regular and magnum masses (See WLP down below).
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We retain it cool inside our hazmat equipment all around beneath I choose to advocate buying a bunch and certain combining it Together with the powder get.
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Typical sequences of Small DNA oligonucleotides are sometimes utilised as primers for sequencing cloned genes (Sanger sequencing). Choose between our typically employed sequencing primers to initiate your sequence verification projects.
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